Primer

Part:BBa_K5007009:Design

Designed by: Pedro Ribeiro Faria   Group: iGEM23_USP-EEL-Brazil   (2023-10-06)


CBD_sub F-primer


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 6
    Illegal PstI site found at 12
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 6
    Illegal PstI site found at 12
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 6
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 6
    Illegal PstI site found at 12
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 6
    Illegal PstI site found at 12
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Primer designed for cloning part BBa_K5007007 into the pRS426 plasmid through Gibson Assembly, allowing for white-blue screening. The USP-EEL-Brazil team will be working to optimize the assembly protocols.



Source

This primer was designed internally and synthesized for use in PCR reactions.

References